Peruskonseptit
Geneexpressiondatacontainsinformationaboutgeneactivity,whichcanreflectthecurrentphysiologicalstateofcells,suchaswhethercellsareinanormalordeterioratingstate,whetherdrugsareeffectiveontumorcells,etc..Theanalysisofgeneexpressiondatacanobtaingenefunctionandgeneexpressionregulationinformation.ThisisoneofthemajorchallengesofbioinformaticsandoneofthekeyreasonswhyDNAmicroarrayscanbewidelyusedinthefieldofbiomedicine.
Ominaisuudet ja tutkimusohjeet
ominaisuudet
Theobjectofgeneexpressiondataanalysisisadatamatrixcomposedofallorpartofthegeneexpressiondataunderdifferentconditions.Throughtheanalysisofthisdatamatrix,somebiologicalquestionscanbeanswered.Theanswerstothesequestions,combinedwithotherbiologicalknowledgeanddata,willhelpclarifygeneexpressionregulationpathwaysandregulatorynetworks.Revealinggeneregulationpathwaysandnetworksisthecommongoalofbiologyandbioinformatics,andisthecorecontentofsystemsbiologyresearch.
Tutkimuksen suunta
1.Analysoiilmaisuntaso
Accordingtothechangeofgeneexpressionlevelunderdifferentexperimentalconditions,Tojudgeitsfunction,forexample,thespecificgenesrelatedtotumortypingcanbedeterminedbasedonthesignificanceoftheexpressiondifference.Theanalysismethodsusedincludehypothesistestinginstatisticsandsoon.
2. Harkitse yhdistelmiä
Groupgenestostudytheircommonfunctions,interactions,andcoordinatedregulation.Methodssuchasclusteranalysisareoftenused.
3.Yritä selvittää mahdollinen geenisäätelyverkko
Interprettheobservedgeneexpressiondatafromthemechanism.Reverseengineeringmethodsareoftenused.