Основни понятия
Geneexpressiondatacontainsinformationaboutgeneactivity,whichcanreflectthecurrentphysiologicalstateofcells,suchaswhethercellsareinanormalordeterioratingstate,whetherdrugsareeffectiveontumorcells,etc..Theanalysisofgeneexpressiondatacanobtaingenefunctionandgeneexpressionregulationinformation.ThisisoneofthemajorchallengesofbioinformaticsandoneofthekeyreasonswhyDNAmicroarrayscanbewidelyusedinthefieldofbiomedicine.
Характеристики и насоки за изследване
Характеристика
Theobjectofgeneexpressiondataanalysisisadatamatrixcomposedofallorpartofthegeneexpressiondataunderdifferentconditions.Throughtheanalysisofthisdatamatrix,somebiologicalquestionscanbeanswered.Theanswerstothesequestions,combinedwithotherbiologicalknowledgeanddata,willhelpclarifygeneexpressionregulationpathwaysandregulatorynetworks.Revealinggeneregulationpathwaysandnetworksisthecommongoalofbiologyandbioinformatics,andisthecorecontentofsystemsbiologyresearch.
Изследователска посока
1. Анализирайте нивото на експресия на един ген
Accordingtothechangeofgeneexpressionlevelunderdifferentexperimentalconditions,Tojudgeitsfunction,forexample,thespecificgenesrelatedtotumortypingcanbedeterminedbasedonthesignificanceoftheexpressiondifference.Theanalysismethodsusedincludehypothesistestinginstatisticsandsoon.
2. Обмислете комбинации
Groupgenestostudytheircommonfunctions,interactions,andcoordinatedregulation.Methodssuchasclusteranalysisareoftenused.
3. Опитайте се да разберете потенциалната мрежа за регулиране на поколенията
Interprettheobservedgeneexpressiondatafromthemechanism.Reverseengineeringmethodsareoftenused.