Kurt Counting Method

Basic principle

The Coulter counter measurement method is to treat somatic cells as particles, set a critical level in the instrument, and at the same time make the body fine... The electronic counter is equipped with a 100μm capillary, and its counting volume can be divided into 0.1 or 0.5mL according to the model (model F or FN2).

The counter should be calibrated before use to determine the number of somatic cells.

Measuring method

Method for measuring particle and powder particle size: Coulter counting method is to fill an electrolyte solution in a measuring tube, and suspend the particles in the electrolyte solution. There is a fine hole on the wall of the measuring tube, and there is a certain voltage between the hole electrodes. When the particles pass through the fine hole, the electric current changes due to the change in resistance and is recorded on the recorder. Finally, the electrical signal can be converted into particle size. This method can be used to obtain the particle size distribution.

This method can be used to determine the particle size distribution of suspensions, emulsions, liposomes, powdered medicines, etc.

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